Journal: Cell reports

Article Title: Modeling Progressive Fibrosis with Pluripotent Stem Cells Identifies an Anti-fibrotic Small Molecule

doi: 10.1016/j.celrep.2019.11.019

Figure Lengend Snippet:

Article Snippet: Rabbit p-SMAD2/3 , R and D systems , Cat# MAB8935; RRID:AB_2313773.

Techniques: Virus, Recombinant, Luciferase, Imaging, Enzyme-linked Immunosorbent Assay, Hydroxyproline Assay, Software

Journal: Cellular microbiology

Article Title: Plasmodium falciparum soluble extracts potentiate the suppressive function of polyclonal T regulatory cells through activation of TGFβ-mediated signals.

doi: 10.1111/j.1462-5822.2011.01622.x

Figure Lengend Snippet: Fig. 6. Effect of antibodies to P. falciparum TRAP and MMP inhibitor on TGFb signalling of CD25+ cells. Activated CD25+ cells were incubated in serum-free medium for 2 h in the presence of 10 mg ml-1 PfSEs (A) or uRBC lysates (B), pre-treated with mouse monoclonal anti-PfTRAP antibodies (1 mg ml-1) or anti-human TSP antibodies (1 mg ml-1) or control mouse IgG (1 mg ml-1) or GM 1489 (0.2 mg ml-1) or the inhibitor vehicle DMSO. Western blot analysis was performed by using anti-phospho-SMAD2/3 and anti-total SMAD 2/3 antibodies. The histograms represent the mean increase of SMAD 2/3 phosphorylation induced by PfSEs or uRBCs stimulation relative to unstimulated cultures in the presence of different inhibitors. Data from each experimental point were recorded as P-SMAD/T-SMAD. Statistical analysis was performed by Student’s t-test.

Article Snippet: Membranes were stripped at 56°C and stained with 1:1000 a-total SMAD2 (Cell Signaling, Cambridge, UK).

Techniques: Incubation, Control, Western Blot, Phospho-proteomics