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Image Search Results
Journal: Cell reports
Article Title: Modeling Progressive Fibrosis with Pluripotent Stem Cells Identifies an Anti-fibrotic Small Molecule
doi: 10.1016/j.celrep.2019.11.019
Figure Lengend Snippet:
Article Snippet:
Techniques: Virus, Recombinant, Luciferase, Imaging, Enzyme-linked Immunosorbent Assay, Hydroxyproline Assay, Software
Journal: Medical Science Monitor : International Medical Journal of Experimental and Clinical Research
Article Title: Puerarin Attenuates Cardiac Hypertrophy Partly Through Increasing Mir-15b/195 Expression and Suppressing Non-Canonical Transforming Growth Factor Beta (Tgfβ) Signal Pathway
doi: 10.12659/MSM.895877
Figure Lengend Snippet: Primer sequences for qRT-PCR.
Article Snippet: The ready-to-use Smad2/3 and
Techniques:
Journal: Medical Science Monitor : International Medical Journal of Experimental and Clinical Research
Article Title: Puerarin Attenuates Cardiac Hypertrophy Partly Through Increasing Mir-15b/195 Expression and Suppressing Non-Canonical Transforming Growth Factor Beta (Tgfβ) Signal Pathway
doi: 10.12659/MSM.895877
Figure Lengend Snippet: Puerarin suppresses the canonical and non-canonical TGFβ signal pathways partially through miR-15b and miR-195. ( A ) QRT-PCR analysis of the TGFβ receptors (TGFBR1, TGFBR2 and TGFBR3), canonical (Smad2, Smad3, Smad4, and Smad7) and non-canonical TGFβ signal members (TAK1 and p38) in the ventricular tissues in the sham, Ang II-infused, and puerarin groups. ( B ) Western blot analysis of Smad2, Smad3, Smad4, Smad7, TAK1, and p38 expression in the groups indicated in Figure A. ( C ) QRT-PCR analysis of miR-15 family expression in the primary cardiomyocytes after treatment with puerarin. ( D, E ) QRT-PCR analysis of miR-15b and miR-195 expression in the primary cardiomyocytes after infection with the pLV-miR-15b and pLV-miR-195 expression ( D ) or the pLV-miR-15b and pLV-miR-195 locker lentiviral particles ( E ). ( F, G, H ) QRT-PCR analysis Smad2, Smad3, Smad4, Smad7, and p38 expression in the primary cardiomyocytes with miR-15b or miR-195 overexpression ( F ) or miR-15b or miR-195 knockdown ( G ) or after treatment with puerarin with or without knockdown of endogenous miR-15b and miR-195 ( H ). * Comparison with NC group; # comparison with puerarin group. * and # p<0.05, ** and ## p<0.01, *** and ### p<0.001.
Article Snippet: The ready-to-use Smad2/3 and
Techniques: Quantitative RT-PCR, Western Blot, Expressing, Infection, Over Expression, Knockdown, Comparison